Journal: Molecular Plant Pathology

Article Title: Transgenic expression of pectin methylesterase inhibitors limits tobamovirus spread in tobacco and A rabidopsis

doi: 10.1111/mpp.12090

Figure Lengend Snippet: Detection of recombinant Turnip vein clearing virus (TVCV)‐MPHis after nickel affinity column chromatography by sodium dodecylsulphate‐polyacrylamide gel electrophoresis (SDS‐PAGE) (a) and Western blot analysis (b). M, molecular weight marker; lane 1, proteins from Escherichia coli M15 (negative control); lane 2, proteins from E. coli M15 transformed with TVCV‐MPHis. Arrows indicate the purified movement protein (MP). Pectin methylesterase (PME) activity in fractions eluted from affinity chromatography on a Sepharose column conjugated with TVCV‐MPHis (c) and from a blank Sepharose column (d): 1, intercellular washing fluids; 2, flow‐through; 3, washing; 4, proteins eluted from the columns.

Article Snippet: Tobamovirus infection of tobacco and A rabidopsis plants TVCV (strain PV‐0361) (DSMZ GmbH, Braunschweig, Germany) and TMV (strain U1) were propagated in Arabidopsis and tobacco plants, respectively, and purified as described by Gooding and Hebert ( 1967 ) and Lartey et al . ( 1993 ).

Techniques: Recombinant, Virus, Affinity Column, Chromatography, Polyacrylamide Gel Electrophoresis, SDS Page, Western Blot, Molecular Weight, Marker, Negative Control, Transformation Assay, Purification, Activity Assay, Affinity Chromatography

Journal: Molecular Plant Pathology

Article Title: Transgenic expression of pectin methylesterase inhibitors limits tobamovirus spread in tobacco and A rabidopsis

doi: 10.1111/mpp.12090

Figure Lengend Snippet: Systemic accumulation of Turnip vein clearing virus‐coat protein (TVCV‐CP) in rosette leaves of wild‐type (WT) and pectin methylesterase inhibitor‐overexpressing Arabidopsis (AtPMEI‐2) plants. (a) Representative sodium dodecylsulphate‐polyacrylamide gel electrophoresis (SDS‐PAGE) indicating TVCV‐CP accumulation (arrow) in systemic leaves of infected WT and AtPMEI‐2 plants at the indicated days post‐inoculation. (b) CP accumulation is expressed as a percentage of the maximal amount of CP measured in WT plants. Data represent average ± standard error (n = 3). The experiment was repeated three times with similar results.

Article Snippet: Tobamovirus infection of tobacco and A rabidopsis plants TVCV (strain PV‐0361) (DSMZ GmbH, Braunschweig, Germany) and TMV (strain U1) were propagated in Arabidopsis and tobacco plants, respectively, and purified as described by Gooding and Hebert ( 1967 ) and Lartey et al . ( 1993 ).

Techniques: Virus, Polyacrylamide Gel Electrophoresis, SDS Page, Infection

Journal: Molecular Plant Pathology

Article Title: Transgenic expression of pectin methylesterase inhibitors limits tobamovirus spread in tobacco and A rabidopsis

doi: 10.1111/mpp.12090

Figure Lengend Snippet: Pectin methylesterase (PME) activity in wild‐type (WT) and transgenic Arabidopsis plants at early stages of Turnip vein clearing virus (TVCV) infection. PME activity was quantified at the indicated hours post‐inoculation (hpi); M, mock‐inoculated plants; TVCV, virus‐inoculated plants. Data represent the average ± standard deviation (n = 6). The experiment was repeated twice with similar results. The different letters indicate datasets significantly different according to analysis of variance (ANOVA) followed by Tukey's test (P < 0.05).

Article Snippet: Tobamovirus infection of tobacco and A rabidopsis plants TVCV (strain PV‐0361) (DSMZ GmbH, Braunschweig, Germany) and TMV (strain U1) were propagated in Arabidopsis and tobacco plants, respectively, and purified as described by Gooding and Hebert ( 1967 ) and Lartey et al . ( 1993 ).

Techniques: Activity Assay, Transgenic Assay, Virus, Infection, Standard Deviation